- Techniques for the detection and characterization of microbial pathogens in foods and environmental samples
- Molecular techniques and their underlying theories and protocols laid out step by step
- Protocols cover: sample processing, DNA extraction, PCR amplification of target genes, methods for quantifying and quality checking DNA/RNA samples
- Also presents conventional microbiology, DNA, RNA and other genetic techniques using commercially available materials and kits
- Guidance for examining, recording, and interpreting data
Combining the depth of a textbook and the guidance of a lab manual, this volume introduces contemporary molecular methods that are widely used to identify and characterize microbial populations and specific pathogens. The book is structured to explain the basic theory and the rationale for choosing appropriate approaches and specific methods. This material is supplemented by in-depth descriptions of lab experiments. Step-by-step guidelines are provided for a variety of experimental objectives. The techniques explained in this volume can be used in a variety of instructional settings and have been taught to students in academic disciplines such as food science, nutrition, environmental technology, and animal science.
I. BATA-VIDÁCS – :
Though traditional microbiological methods, as for example culture media-based techniques, are still essential in the detection, enumeration, and characterisation if microbes, there is a growing demand for methods based on molecular biology. Today, students aquire the skills to perform molecular analyses through independent laboratory research, but textbooks providing step-by-step protocols for these molecular methods are rare. The book “Molecular methods for the detection and characterization of foodborne and environmental pathogens” fills this gap.
The book contains six chapters: “Microorganisms: Detection and characterization”, “Microbial DNA extraction, purification, and quantification”, Introduction to PCR amplification”, DNA fingerprinting”, Mictobial RNA extraction, purification, and quantification”, and “Gene expression using real-time PCR assay”. As a very useful feature, all chapters have a “Laboratory experiments” section, where detailed protocols and instructions are presented for the everyday laboratory work.
Based on a graduate level course titled “Molecular methods of microbial detection and characterisation” that Professor Pillai teaches at Texas ACM University, the book targets senior undergraduate and graduate-level students, lecturers, instructors, and all microbiologists who want to keep up with the progress of new technologies.